Poehler, 38, who married the Arrested Development star in August 2003 -- previously welcomed son Archie in October 2008.
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"I've been very lucky," Poehler told People at the time. "I’m feeling good, and I’m just overwhelmed with blessings."
Poehler will be back for a third season of NBC's "Parks & Recreation." the early season three pickup was designed to accommodate Amy's pregnancy.
"It will not affect the show," a source told E! Online in March. "They will take a short break and then resume production."